首页 ??BA/F3_EGFR L858R-T790M-C797S cell line细胞株细胞系 BioVector NTCC质粒yabo88ios菌种细胞基因保藏中心

BA/F3_EGFR L858R-T790M-C797S cell line细胞株细胞系 BioVector NTCC质粒yabo88ios菌种细胞基因保藏中心

  • 价  格:¥3986840
  • 货  号:BA/F3_EGFR L858R-T790M-C797S cell line细胞株细胞系
  • 产  地:北京
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BA/F3_EGFR L858R-T790M-C797S cell line细胞株细胞系 BioVector NTCC质粒yabo88ios菌种细胞基因保藏中心

Cell Line Name


Ba/F3_EGFR-L858R/T790M/C797S


Gene Synonyms:


Epidermal Growth Factor Receptor, ERBB1,EGFR


Host Cell:


Ba/F3


Stability:16passages

Application:


Anti-proliferation assay and PD assay


Freeze Medium:


90% FBS+10% DMSO


Complete Culture Medium:


RPMI-1640+10% FBS+1 ug/ml puromycin


Mycoplasma Status:


Negative


II.Background

EGFR is widely recognized for itsimportance in cancer. Amplification and mutations have been shown to be drivingevents in many cancer types. Its role in non-small cell lung cancer,glioblastoma and basal-like breast cancers has spurred many research and drugdevelopment efforts. Tyrosine kinase inhibitors have shown efficacy in EGFRamplfied tumors, most notably gefitinib and erlotinib. Mutations in EGFR havebeen shown to confer resistance to these drugs, particularly the variant T790M,which has been functionally characterized as a resistance marker for both ofthese drugs. The later generation TKI's have seen some success in treatingthese resistant cases, and targeted sequencing of the EGFR locus has become acommon practice in treatment of non-small cell lung cancer. Overproduction ofligands is another possible mechanism of activation of EGFR. ERBB ligands includeEGF, TGF-a, AREG, EPG, BTC, HB-EGF, EPR and NRG1-4 (for detailed informationplease refer to the respective ligand section). In ligand-activated cancers,Cetuximab appears to be more effective than tyrosine-kinase inhibitors.


III. Representative Data

1. WB of EGFR-L858R/T790M/C797S expression



Figure 1. WB of EGFR Expression

Lane 1: Negative control

Lane 2: EGFR-WT

Lane 3: EGFR-L858R/T790M

Lane 4: EGFR-L858R/T790M/C797S


2. Sanger sequencing



Figure 2. Sanger Sequencing ofEGFR-L858R/T790M/C797S


3. Anti-proliferation assay




Figure 3. Anti-proliferation assay of threereference compounds on the Ba/F3_EGFR-L858R/T790M/C797S Stable Cell Line




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