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发布时间:10-20 23:51

基因cDNA,全长ORF,克隆质粒、原核/真核过表达质粒、荧光标签表达质粒、基因沉默RNAi质粒、CRISPR/Cas9基因敲除质粒、腺病毒逆病毒慢病毒yabo88ios及病毒毒液、酵母表达质粒、昆虫表达质粒,麦胚无细胞表达质粒等,包括人源,鼠源,模式生物基因


cDNA,全长ORF及其表达质粒(基因名称以Gene”代替):pCMV-Gene,pcDNA3.1-Gene,标签表达质粒pCMV-myc/Flag-Gene,荧光标签质粒pCMV-Gene-EGFP, 慢病毒质粒pLenti-CMV-Gene,克隆质粒pDONR223-Gene, pOTB7-Gene,以及该基因的原核表达质粒pET-Gene, 酵母表达质粒pADH-Gene-GAL4AD、昆虫表达质粒pPH-His-Gene,基因沉默质粒pSilencer-Gene、启动子报告质粒pGene Promoter-EGFP等,现货当日可发送;相关图谱序列及酶切位点信息请发邮件查询。

另有该基因克隆质粒/表达质粒/启动子报告质粒/腺病毒慢病毒逆病毒RNAiyabo88ios由Biovector Inc.现货供应,具体查询http://www.Biovector.net包括如下:

基因真核表达质粒

pCMV-GENE,

pCMV-GENE-Flag,

pcDNA3.1-GENE,

pCMV-His-GENE,

pCMV-Myc-GENE,

pCMV-GST-GENE,

pCMV-HA-GENE,

pCMV-SPORT6-GENE,

pCMV6-GENE

基因真核荧光表达质粒

pCMV-GENE-EGFP,

pEGFP-GENE,

pCMV-mCherry-GENE,

pCMV-ECFP-GENE,

pCMV-EYFP-GENE,

pCMV-Luc-GENE,

基因真核表达yabo88ios特点:

携带CMV启动子,可在真核细胞进行表达;

带T7启动子,可转化大肠杆菌,进行原核蛋白表达;

有融合N、C端EGFP, Flag, His, myc, HA等标签的质粒,可用于蛋白纯化、检测及跟踪;

含有Neomycin, puromycin等筛选标记,可用于构建稳定细胞株。

基因慢病毒yabo88ios

pLenti-GENE

[LVX-GENE

pLenti-CMV-GENE

pLenti-CMV-EGFP-GENE

pLenti-CMV-GENE-puro,

pLenti-CMV-GENE-V5,


基因原核表达质粒

pET-GENE,

pGEX-GENE,

pMAL-GENE,

pTac-GENE-GST,

pTac-GENE-MBP,

pET-His-GENE,

pT7-GENE-HisSUMO,

pBAD-GENE,

pQE-GENE,

pGEX-GENE-GST,

基因酵母表达质粒

pYES2-GENE,

pYES3-GENE,

pPIC9k-GENE,

pPICZa-GENE,

pPIC3.5k-GENE,

pGAL1-GENE,

pADH-GENE-GAL4AD,

pADH-GENE-GAL4BD,

基因昆虫细胞表达yabo88ios

pPH-His-GENE,

pIZT/V5-His-GENE,

pMIB v5-His-GENE,

pFastBac-GENE,

pBlueBacHis-GENE,

Wheet Germ Cell Free(麦胚无细胞体系) 表达质粒

pEXP-GENE,

pT7-His-GENE,

Gateway兼容的克隆质粒pDONR223-GENE,pENTR-GENE,

克隆质粒pOTB7-GENE,pBluescriptR-GENE,pCMV-SPORT6-GENE,pCR-TOPO-GENE,

全长转录本 cDNA 克隆 (含 5' 和 3' UTRs),

基因沉默RNAi(shRNA,siRNA,miRNA)yabo88iospSilencer-Gene, pSIREN-reteoQ-Gene,

启动子报告质粒pGenePromoter-GLuc,

pGene Promoter-GLuc-CMV-SEAP,

pGene Promoter-EGFP,

pGene Promoter-mCherry,

基因启动子报告慢病毒质粒pLenti-Promoter-GLuc,等;

以上现货当日可发送;

另可根据您的需要选择yabo88ios骨架定制表达质粒,一周左右完成交付。


稀有基因合成、稀有质粒yabo88ios合成

稀缺稀有基因合成/模式生物基因克隆合成,基于引进的新一代合成平台3.0系统,合成速度颠覆性加快,更加准确,价格更低.显着降低您的基因合成成本,提高工作效率,并享有自主知识产权,不会因购买克隆而产生工业化应用的障碍。

请提交您的基因序列发送至Biovector@163.com

我们的技术人员分析序列后马上回复您合成所需时间和报价。


自选yabo88ios骨架:


哺乳动物表达yabo88iospcDNA3.1+/-,pcDNA4/HisMax BpSecTag2 ApVAX1pBudCE4.1,pTracer CMV2pcDNA3.1(-)/myc-HisA pcDNA6-Myc/His B pCEP4 pIRESpIRESneopIRES hyg3pCMV-mycpCMV-HApIRES-puro3pIRES-neo3,pCAGGS哺乳动物双杂交系统pACTpBINDpACT-MyoDpBIND-IdpG5luc,pCMV-BD, pCMV-AD, pBD-p53, pFR-luc,CytotrapTwo-Hybrid System:pSos, pSos MAFB, pMyr蜕皮激素诱导系统pIND, pVgRxR,LacSwith II哺乳动物诱导表达系统:pOPRSVI pOPI3CATpCMVLacI,GeneSwitch System:pSwitch哺乳动物表面展示系统:pDisplay, 四环素调控系统(Invitrogen):pcDNA4/TO/Myc-His ApcDNA4/TO/Myc-HisBpcDNA4/TO/Myc-His CpcDNA4/TO/Myc-His/LacZpcDNA6/TR四环素调控系统(Clontech):pTet-OnpTet-OffpTRE2,pRevTREpRevTet-OnpRevTet-off信号通路报告yabo88ios:pGAS-TA-LucpSTAT3-TA-Luc, pISRE-TA-Luc, pTA-LucpIκB-EGFPpNFAT-TA-LucpCaspase3-sensorpAP1(PMA)-Luc;pGL4.26[luc2P/minP/Hygro],pGL4.29[luc2P/CRE/Hygro],pGL4.30[luc2P/NFAT-RE/Hygro]pGL4.75;p53-LucpAP-1-Luc pNF-κB-LucpSRE-LucpFA2-Elk1pFC-MEKKpFR-luc,Gateway系统(invitrogenpcDNA6.2-GWEmGFP-miR negative, pLenti 6/TR,pcDNA 6.2-GWEmGFP-miR

标签:Biovector Inc. 质粒 yabo88ios 索取 报价 价格 优惠售价 出售 图谱 序列 酶切位点 转化

酿酒酵母表达yabo88ios

pYES2,pYES2/NT,pYES2/CT,pYES3,pYES6,pYCplac22-GFP,酵母yabo88iospAUR123,pRS303TEF,pRS304,pRS305,pRS306,pY13TEF,pY14TEF,pY15TEF,pY16TEF,酵母基因重组表达yabo88iospUG6, pSH47,酵母单杂yabo88iospHISi,pLacZi,pHIS2, pGAD424, 酵母双杂交系统:酿酒酵母Y187,酿酒酵母AH109;质粒pGADT7,pGBKT7;对照质粒pGBKT7-53,pGBKT7-lam,pGADT7-T,PCL1,酿酒酵母菌株INVSc1,YM4271, AH109,Y187,Y190,

毕赤酵母表达yabo88ios

pPIC9K,pPIC9K-His,pPIC3.5K,pPICZalphaA,B,C,pPICZA,B,C,pGAPZαA,pAO815,pPIC9k-His,pHIL-S1,pPinkhc,配套毕赤酵母Pichiapink1,2,3,4

毕赤酵母宿主菌

X33,KM71,KM71H,GS115,SMD1168, SMD1168H,SMD1163,PicheaPink strain1,2,3,4

原核表达yabo88iospQE30,31,32,40,60,61,62,等原核表达yabo88ios,包括pET系列,pET-GSTpGEX系列(GST标签)pMAL系列pMAL-c2x,-c4x,-c4e,-c5x,-p5x,pBAD,pBADHis,pBADmycHis系列,pQE系列,pTrc99a,pTrcHis系列,pBV220,221,222,pTXB系列,pLLP-ompA,pIN-III-ompA(分泌型表达系列),pQBI63(原核表达带荧光)pET3a, pET 3d, pET 11a, pET 12a, pET 14b, pET 15b, pET16b, pET 17b, pET 19b, pET 20b, pET 21a,b,d, pET 22b, pET 23a, pET 23b, pET24a,b, pET 25b, pET 26b, pET 27b, pET 28a,b, pET 29a, pET 30a, pET 31b, pET32a, pET 35b, pET 38b, pET 39b, pET 40b, pET 41a,b pET 42a, pET 43.1a,b pET44a, pET 49b pET302,303 pET His,pET Dsb,pET GST,pET Trx pQE2, pQE9 pQE30,31,32,pQE 40 pQE70 pQE80L pQETirs system pRSET-A pRSET-B pRSET-CpGEX4T-1,-2,-3,5x-1,6p-1,6p-2,2tk,3c pBV220,221,222 pTrcHisA,B,C pBAD24,34,43pBAD HisA,B,C pPinPoint-Xa1,Xa2,Xa3 pMALc2x, p2x pBV220 pGEM Ex1, pGEM7ZF(+),pTrc99A, pTwin1, pEZZ18 pkk232-8,pkk 233-3,pACYC184,pBR322,pUC119pTYB1,pTYB2,pTYB4,pTYB11 pBlueScript SK(+),pBlueScript SK(-) pLLP ompA,pINIIIompA, pMBP-P ,pMBP-C, 大肠杆菌冷激质粒: pColdI pColdIIpColdIII pColdTF 原核共表达质粒:pACYCduet-1,pETduet-1,pCDFduet-1pRSFduet-1 Takara公司大肠杆菌分子伴侣: pG-KJE8 pGro7 pKJE7 pGTf2 pTf16 大肠杆菌宿主细胞: DH5a JM101 JM103 JM105 JM107 JM109 JM110 Top10 Top10FBL21(DE3) HB101 ER2529 E2566 C2566 MG1655 XL-10gold XL blue M15 JF1125 K802SG1117 BL21(AI) BL21(DE3)plysS TG1 TB1 DH5apir Tuner(DE3) Bl21 codonplusRIPL Novablue(DE3) RosettaRosetta(DE3) Rosetta(DE3)plys Rosetta-gami(DE3) Rosetta-gamiB(DE3),Rosetta-gamiB(DE3)plysS Orgami(DE3) OrgamiB(DE3) HMS174(DE3)

植物表达/RNAiyabo88ios农杆菌pBI121,pBI121-GFP,pBI101,pBI221,pSN1301,pUN1301,pRTL2 , pRTL2-GFP , pRTL2-CFP, pRTL2-RFP , pRTL2-YFP,pCAMBIA 1300,1301, 1302,1303,1304,1305, 1381Z,1391Z,2300, 2301,3300,3301,pCAMBIAsuper1300,pCAMBIA super1300-GFP,pPZP212,pPZP2121,pPZP212-GFP,pGDG,RNAiyabo88iospART27,pHANNIBAL,pKANNIBAL, pFGC5941,pTCK303,pTRV1,pTRV2, T-DNA插入yabo88ios(随机突变体库)pSKI015,pSKI074,真菌ATMTyabo88iospBIG2RHPH2-GUS-GFP,pBHt1


枯草芽孢杆菌表达yabo88iospWB980,pHT43,pHP13,pHP43,pBE2,pMUTIN4,pUB110,pE194,pMA5, pMK3,pMK4,pHT304,pHY300PLK,pBest502,pDG1363,pSG1154,pAX01, pSAS144,pDL,pDG148-stu,pDG641,pAL12,pUCX05-bgaB,pHT01配套菌株BS168,WB600,WB800,WB700, WB800N,1012,FZB42,1A747,广宿主质粒pVLT33

RNAi基因沉默干扰敲除yabo88iospSilencer1.0pSilencer 2.1-U6 hygropSilencer 3.1-H1 hygropSilencer 3.1-H1 neo pSilencer 4.1-CMV neopSilencer 4.1-CMV puro pMIR-REPORT Luciferase RNAiyabo88ios(oligoengine pSuper-puro RNAi逆转录病毒yabo88ios(clontech): RNAi-Ready pSIREN-Retro Q RNAi-Ready pSIREN-RetroQ-ZsGreenLuciferaseshRNA Annealed Oligonucleotide RNAi慢病毒yabo88ios(addgene): pLKO.1


基因cDNA文库ORF表达克隆质粒,

拥有目前中国最大的cDNA克隆库、覆盖10万人、鼠全长基因的cDNA克隆和超过25,000ORF克隆表达yabo88ios。



悬浮细胞表达系统、DHFR、GS加压筛选表达系统、疫苗/抗体蛋白表达系统、工业级大规模抗体药物蛋白表达:

谷氨酰胺合成酶加压筛选表达系统GS expression system vectorspEE12.4, pEE6.4, 配套CHOK1SV细胞株,

GS表达yabo88iospGP3, pGP3Due, pGP3Con, 配套cGMP banking record cell line,

DHFR筛选表达系统yabo88iospSV2-DHFR,pCI-DHFR, pcDNA4-DHFR, pGP2, pGP2Due, pGP2Con,

高效重组蛋白表达yabo88iospTT5,配套细胞株293EBNA cell line.

疫苗/抗体表达yabo88iospCEP4,现货当日可发送

乳酸菌表达yabo88ios及各种乳酸菌乳酸杆菌菌株pNZ8148,pLEISS,pMG36e,pBBR1MCS-5,pBBR1MCS-6,pRV610,pLEM415,pHY300PLK,分泌型乳酸菌表达yabo88iospVE5523pPG611.1,pPG612.1等和乳酸杆菌菌株宿主菌NZ9000,MG1363,Lactobacilluscasei 1.539,Lactobacillus casei,acidophilus NCFM,1.2,Lactobacillus sakei 23K,L.plantarum,L.rhamnosusGG,B.coagulans,Bifidobacterium bifidum,Bifidobacterium infantis,Lactococcuslactis M17,1663,Lactobacillus reuterii

广宿主表达yabo88ios链球菌表达敲除yabo88ios假单胞菌表达yabo88iospVLT33,pBBR1MCS-2,3,4,5,6,pJRD215,pJN105,pME6032,Cosyabo88iospLAFR3,pMP2444(GFP),pHY300PLK,pRT102,pRL1063a, 转座子yabo88iospUT-miniTn5,pMGS100,pWHM10,pKC1139,pSET152, pOJ260,pPG611.1,pPG612.1

腺病毒yabo88ios/慢病毒,逆转录病毒表达yabo88ios及包装包膜质粒腺病毒系统(Stratagene): pAdEasy-1,pShuttle-CMV,pShuttle,pAdTrack,pAdTrack-CMV, pShuttle-IRES-hrGFP-1、pShuttle-IRES-hrGFP-2、pShuttle-CMV-lacZ,pShuttle-CMV-EGFP-C,pXC1, pBHGE3, 配套大肠杆菌BJ5183,293,293Tcell line 腺相关病毒系统(Stratagene): pAAV-MCS,pAAV-RC,pHelper,pAAV-LacZ,pAAV-IRES-hrGFP,pCMV-MCS,

慢病毒/逆转录病毒表达yabo88ios,RNAi沉默yabo88ios

SBI第三代慢病毒yabo88ios-基于HIV-1的慢病毒yabo88ios系统

pCDH-CMV-MCS-EF1-copGFP

pCDH-CMV-MCS-EF1-puro

pCDH-CMV-MCS-EF1-GFP-T2A-Puro

pCDH-EF1-MCS-T2A-Puro

pCDH-MCS-T2A-Puro-MSCV

pCDF1-MCS2-EF1-copGFP

带荧光标签慢病毒表达yabo88iospLEGFP-C1, pLEGFP-N1,pLVX-DsRed-Monomer-N1,pLVX-AcGFP1-N1, pLVX-AcGFP1-C1, pLenti6/v5-EDST-EGFP, FUGW, pLentilox 3.7,

pLVX-IRES-tdTomato

pLVX-IRES-mCherry

pLVX-IRES-ZsGreen1,

另有慢病毒表达yabo88iospLVX-IRES-neo,

pLVX-IRES-puro,pLVX-puro,

Gateway慢病毒表达yabo88iospLenti4 TO/V5 DEST,pLenti6/v5-EDST,pLenti6/UbC/v5-DEST,

慢病毒RNAiyabo88ios(Gateway兼容)pSLIK-Neo

四环素诱导慢病毒表达yabo88iospLVX-Tight-Puro,pLVX-Tet-On-Advanced,pLVX-TRE 3G, pLVX-Tet 3G, pLenti-CMVTight-eGFP-Neo

病毒包装:二质粒包装系统或三质粒包装系统(新的包装系统):

二质粒系统一:
psPAX2
pMD2.G

二质粒系统二:
pCMV-dR8.2 dvpr
pCMV-VSVG

三质粒包装系统具有更高的包装效率:
pMDLg/pRRE
pRSV-Rev

pMD2.G

逆病毒包装yabo88ios:

pCMV-Gag-pol

pCMV-VSV-G

pCL Ampho

另有病毒表达yabo88ios

pLKO.1,pLKO.1-puro,pLKO.3G,pLVTHM,pLKO.1-EGFP,pLVX-DsRed-Monomer-N1,pLVX-IRES-ZsGreen1,pLVX-AcGFP1-N1,pLenti6/v5-EDST-EGFP,pLenti4 TO/V5 DESTpLenti6/v5-EDST,pLenti6/UbC/v5-EDST,pBABE-puro-EGFP,pBABE-puro,pBABE-Neo,pWPXL, FUGW,pLentilox3.7,RNAi-Ready pSIREN-Retro Q, RNAi-Ready pSIREN-Retro Q-ZsGreen,pSUPER.Retro-GFP/Neo,pSUPER-Retro-Neo, pSUPER.Retro-puro,PLNCX

pLVX-Tight-Puro,pLVX-Tet-On-Advanced,pLVX-IRES-neo

pLVX-IRES-puro,pLVX-puro,

pLVX-shRNA1Clontech 慢病毒yabo88ios
pLVX-shRNA2 Clontech 慢病毒yabo88ios
pLVX-DsRed-Monomer-N1 Clontech 慢病毒yabo88ios
pLVX-IRES-tdTomato Clontech 慢病毒yabo88ios
pLVX-IRES-ZsGreen1 Clontech 慢病毒yabo88ios
pLVX-AcGFP1-N1 Clontech 慢病毒yabo88ios

PLNCX2

pMSCV-HYG

pMSCV-neo

pMSCV-puro

pLEGFP-C1

pLOX-CW-CRE

pLOX-GFP-IRES-TK

pRetroX-IRES-DsRedExpress,pLVX-IRES-mCherry质粒yabo88ios。


基因重组yabo88ios敲除yabo88ios自杀质粒RED重组系统yabo88iospKD3,pKD13,pKD4,pCP20,pKD46,pCVD442,pk18mobsacB,p2NIL, pMAD(革兰氏阳性菌基因敲除)pBT2,RN4220,pKC1139,pOJ260, pJQ200SK,pSET152,pWHM10,pk18mobsacB, pGOAL17,pGOAL19,基因打靶yabo88iosPL253PL451PL452PL453,配套菌株EL250,EL350,pMLM290,292,KJBAC1,pST1374

荧光蛋白表达yabo88ios荧光定位质粒报告基因yabo88iospEGFP,pEYFP,pECFP,pDsRED,pmCherry,pAcGFP,hrGFP,ZsGreen,pDsRed1-C1, pDsRed1-N1, pDsRED-Monomer-N1, pEYFP-C1, pEYFP-N1,pEGFP-1,pEGFP-C1, pEGFP-N1, pEGFP-N3,pcDNA-EGFP1,pECFP-N1,pECFP-C1, pAcGFP1-1 pDsRED2-ER, pDsRED2-Mito, pDsRED2-Nuc,pDsRED2-Peroxi , pEYFP-Golgi, pEYFP-Mem, pEYFP-ER , pEGFP-Actin,pECFP-ER, pIRES2-EGFP, pLEGFP-C1,pLEGFP-N1,pDsRED-Express-N1,pIRES2-DsRed,pIRES-AcGFP1,pmCherry-C1,N1


带有Luc,mCherry(RFP)萤火虫酶+红色荧光报告基因双荧光的慢病毒yabo88ios:

pLenti-PGK-Luciferase-mCherry

pLenti-RFP-Luc (有CMV,EF1alpha,MSCV,UbC多种启动子可选择)

带有Luc-GFP萤火虫酶+绿色荧光双荧光yabo88ios:

pTRE-neo-GFP-Luc (双荧光,可直接表达,也可慢病毒包装;)

pEF1-GFP-Luc

一句话!

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